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Leveraging Liquid Handling Solutions for Precise Polymerase Chain Reaction

Ensure the Accuracy of Your Automated PCR System with High-Precision Liquid Handling Solutions  

An organism's DNA is called the blueprint of life because it carries the instructions needed for the organism to grow, develop, survive and reproduce. However, analyzing the genetic information encoded inside DNA requires large amounts of this genetic material. So when a process enabling the rapid replication of specific DNA segments was developed, it revolutionized genetics, molecular biology, and medical diagnostics, earning its creators a Nobel Prize in chemistry.

The process in question is known as polymerase chain reaction (PCR). This simple, yet elegant scientific tool involves multiple heating and cooling cycles, using only a small amount of DNA to create millions to billions of copies of a specific DNA section over a relatively short period of time. Owing to the PCR method, it is now much easier to study, analyze and use DNA in various applications, such as genetic testing, forensics, and molecular research.

When automated, it is a powerful research method, offering fast and reliable results. That said, PCR also has its limitations. One major challenge with PCR is its sensitivity to even minute changes in sample and reagent volumes. Even tiny variations in the volume can result in unreliable results, making precise dispensing a crucial challenge in PCR automation.

TriContinent’s precise liquid handling solutions for PCR robots have been designed to provide dependable and precise DNA sample amplification in high-throughput laboratory environments. Thanks to exceptionally low flow rates and minimal pulsation, our syringe pumps and air displacement pipettes guarantee accurate and precise sample delivery.

Application Note

Take a deep dive into the fascinating world of polymerase chain reaction with our comprehensive application note and learn how the right liquid handling solutions can help you make the most of this process. Whether you're a researcher, technician or a forensic scientist, this guide will walk you through the intricacies of PCR, breaking down its individual stages with clarity and precision.
Download our easy-to-understand application note and join us in exploring the exciting possibilities PCR offers in genetics, diagnostics, and beyond.

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FAQs

 

What is PCR and how does it work?

Polymerase chain reaction is a molecular biology technique used to amplify DNA. The process starts with the denaturation of double-stranded DNA, separating it into two single-stranded DNA templates. Then, DNA primers are added, which selectively bind to complementary regions on the template strands through base pairing. 
DNA synthesis begins as a heat-stable DNA polymerase enzyme extends the primers, creating new double-stranded DNA molecules. Through a series of temperature cycles, this process is repeated, resulting in the rapid generation of millions of copies of the target DNA region.

 

How does PCR testing contribute to various applications, including diagnostics and research?

Most people associate PCR with Covid-19 testing, where this molecular technique plays a crucial role in detecting the virus’s genetic material. However, the application of PCR testing extends beyond clinical diagnostics, and includes genetics research, forensics, environmental monitoring and biotechnology, enabling the detection and analysis of specific DNA sequences.

 

What is the role of polymerase in PCR?

In PCR, the polymerase enzyme is responsible for replicating the DNA. Different polymerases are used for various applications, and selecting the right one is crucial for success.

 

How can syringe pumps and air displacement pipettes enhance PCR systems?

Syringe pumps and air displacement pipettes are essential components of PCR systems, providing extremely low flow rates and minimal pulsation, which are critical for accurate and precise sample delivery.
If you are looking for a proven solution for low-volume dispensing, you should check out our range of syringe pumps and liquid handling robots here.

 

Download our application note today and learn how you can improve your PCR processes with precise liquid handling solutions. 

Also visit our website for more information on TriContinent's displacement pipettes to ensure the accuracy of your automated PCR system.